[4] Ayr 2-1 Queen of South [8] 2020-10-17 14:00
Stadium: Somerset Park Round: 1
Referee: Steven McLean Home Manager: Mark Kerr Away Manager: Allan Johnston
Head to Head
| Scotland Championship | 2019/12/28 15:00 | Ayr v Queen of South | L | 1-2 |
| Scotland Championship | 2019/11/09 15:00 | Queen of South v Ayr | L | 3-1 |
| Scotland Championship | 2019/08/24 14:00 | Ayr v Queen of South | W | 1-0 |
| Scotland Championship | 2019/04/09 18:45 | Ayr v Queen of South | W | 1-0 |
| Scotland Championship | 2018/12/29 15:00 | Queen of South v Ayr | D | 1-1 |
| Scotland Championship | 2018/11/10 15:00 | Ayr v Queen of South | D | 1-1 |
| Scotland Championship | 2018/09/01 14:00 | Queen of South v Ayr | L | 5-0 |
| Scotland Championship | 2017/04/08 14:00 | Ayr v Queen of South | L | 0-2 |
Home History
| Scotland League Cup | 2020/10/13 18:45 | Ayr v Annan Athletic | W | 1-0 |
| Scotland League Cup | 2020/10/10 14:00 | Hamilton v Ayr | L | 2-1 |
| Scotland League Cup | 2020/10/06 18:45 | Albion v Ayr | W | 2-5 |
| Scotland Championship | 2020/03/10 19:45 | Dundee v Ayr | L | 2-0 |
| Scotland Championship | 2020/03/07 15:00 | Ayr v Dundee | D | 0-0 |
| Scotland Championship | 2020/03/03 19:45 | Ayr v Dundee Utd | D | 0-0 |
| Scotland Championship | 2020/02/29 15:00 | Alloa v Ayr | W | 0-2 |
| Scotland Championship | 2020/02/25 19:45 | Ayr v Morton | L | 1-2 |
Away History
| Scotland League Cup | 2020/10/13 18:45 | Queen of South v Partick | L | 2-3 |
| Scotland League Cup | 2020/10/10 14:00 | Queen of South v St Mirren | L | 4-6 |
| Scotland League Cup | 2020/10/06 18:45 | Morton v Queen of South | W | 4-6 |
| Scotland Championship | 2020/03/10 19:45 | Inverness CT v Queen of South | L | 3-1 |
| Scotland Championship | 2020/03/07 15:00 | Dunfermline v Queen of South | D | 1-1 |
| Scotland Championship | 2020/03/03 19:45 | Partick v Queen of South | D | 0-0 |
| Scotland Championship | 2020/02/29 15:00 | Queen of South v Morton | L | 0-4 |
| Scotland Championship | 2020/02/22 15:00 | Queen of South v Dundee | L | 0-1 |