Ayano Shimizu (清水 綾乃, Shimizu Ayano, born 11 April 1998) is a Japanese tennis player.
Shimizu has a career-high singles ranking of 175 by the Women's Tennis Association (WTA), achieved in May 2019, and a best WTA doubles ranking of 145, set in October 2018. Shimizu has won six singles and ten doubles titles on the ITF Women's Circuit.
She won her biggest title to date at the 2018 Kurume Cup, defeating Abbie Myers in the final.