| No. | Name | Birth Date | Age | Country | Position | Height | Weight | Preferred foot |
| 43 | David De Gea | 1990-11-07 | 34 | Guard | 192 | 71 | Right | |
| 3 | Cristiano Biraghi | 1992-09-01 | 32 | Defender | 185 | 75 | Left | |
| 1 | Pietro Terracciano | 1990-03-08 | 34 | Guard | 193 | 85 | Right | |
| 10 | Albert Gudmundsson | 1997-06-15 | 27 | Forward | 177 | 80 | Right | |
| 32 | Danilo Cataldi | 1994-08-06 | 30 | Midfielder | 180 | 75 | Right | |
| 21 | Robin Gosens | 1994-07-05 | 30 | Defender | 183 | 81 | Left | |
| 8 | Rolando Mandragora | 1997-06-29 | 27 | Midfielder | 183 | 75 | Left | |
| 11 | Jonathan Ikone | 1998-05-02 | 26 | Midfielder | 175 | 73 | Left | |
| 5 | Marin Pongracic | 1997-09-11 | 27 | Defender | 190 | 95 | Right | |
| 99 | Christian Kouame | 1997-12-06 | 27 | Forward | 185 | 70 | Right | |
| 2 | Dodo | 1998-11-17 | 26 | Defender | 166 | 68 | Right | |
| 20 | Moise Kean | 2000-02-28 | 24 | Forward | 183 | 72 | Right | |
| 7 | Riccardo Sottil | 1999-06-03 | 25 | Forward | 180 | 70 | Right | |
| 29 | Yacine Adli | 2000-07-29 | 24 | Forward | 186 | 78 | Right | |
| 53 | Oliver Christensen | 1999-03-22 | 25 | Guard | 190 | 82 | Right | |
| 6 | Luca Ranieri | 1999-04-23 | 25 | Defender | 187 | 74 | Left | |
| 23 | Andrea Colpani | 1999-05-11 | 25 | Midfielder | 186 | 79 | Left | |
| 65 | Fabiano Parisi | 2000-11-09 | 24 | Midfielder | 178 | 70 | Left | |
| 9 | Lucas Beltran | 2001-03-29 | 23 | Forward | 176 | 77 | Right | |
| 4 | Edoardo Bove | 2002-05-16 | 22 | 181 | 72 | Right | ||
| 14 | Nicolas Valentini | 2001-04-06 | 23 | 187 | 79 | Left | ||
| 24 | Amir Richardson | 2002-01-24 | 22 | 195 | 92 | Left | ||
| 33 | Michael Kayode | 2004-07-10 | 20 | 179 | 74 | Right | ||
| 30 | Tommaso Martinelli | 2006-01-06 | 19 | 194 | 82 | Right | ||
| 40 | Tommaso Vannucchi | 2007-03-05 | 17 | 197 | 90 | Left | ||
| 22 | Matias Moreno | 2003-09-24 | 21 | 193 | 81 | Right | ||
| 15 | Pietro Comuzzo | 2005-02-20 | 19 | 185 | 79 | Right | ||
| 63 | Maat Daniel Caprini | 2006-02-11 | 18 | 180 | 72 | Right | ||
| 64 | Jonas Harder | 2005-09-30 | 19 | 178 | 73 | Right | ||
| 60 | Eddy Kouadio | 2006-05-07 | 18 | 191 | 74 | Right | ||
| 61 | Leonardo Baroncelli | 2005-08-13 | 19 | 182 | 74 | Both | ||
| 66 | Tommaso Rubino | 2006-11-10 | 18 | 179 | 63 | Right |